Inflammatory cytokine mRNA expression during early and persistent Helicobacter pylori infection in nonhuman primates
Keywords: system, animals, infections, expression, cytokine, transcription, monocytes, gene, chain, histopathology, tumor, macrophages, male, polymerase, tissue, gastritis, necrosis, rna, factor-alpha, immune, mucosa, primate, monkey, interleukin-6, article, interleukin-1, factor, controlled, animal, helicobacter, pylori, reverse, gastric, study, response, interleukin, priority, Reaction, nonhuman, journal, Macaca, mulatta, RNA,, Messenger, rhesus, 6, 1beta, Transcriptase, phagocyte
Abstract
The role of mononuclear phagocytes in orchestrating the host responses to Helicobacter pylori is inadequately understood. Therefore, gene expression for the monocyte/macrophage-derived cytokines interleukin (IL)- 1?, IL-6, and tumor necrosis factor (TNF)-? was determined before and during H. pylori infection of rhesus monkeys by use of a highly sensitive quantitative reverse transcriptase polymerase chain reaction. The numbers of molecules of IL-1?, IL-6, and TNF-? mRNA in gastric tissue during early infection (7 weeks) significantly exceeded the preinfection numbers (P < .03). Moreover, the numbers of IL-1?, IL-6, and TNF? mRNA molecules in persistently infected animals (6 years) also were elevated compared with preinfection numbers (P < .02, P = .03, P = .16, respectively). Cytokine gene expression coincided with progressive H. pylori gastritis, confirmed by increased gastritis scores over preinfection scores (P < .005). These findings provide quantitative evidence that H. pylori induces local gene expression of monocyte/macrophage-derived inflammatory cytokines and evokes an innate response in gastric tissue of nonhuman primates.
Más información
Título según SCOPUS: | Inflammatory cytokine mRNA expression during early and persistent Helicobacter pylori infection in nonhuman primates |
Título de la Revista: | JOURNAL OF INFECTIOUS DISEASES |
Volumen: | 181 |
Número: | 2 |
Editorial: | OXFORD UNIV PRESS INC |
Fecha de publicación: | 2000 |
Página de inicio: | 783 |
Página final: | 786 |
Idioma: | English |
URL: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0034088755&partnerID=q2rCbXpz |
DOI: |
10.1086/315257 |
Notas: | SCOPUS |