Inflammatory cytokine mRNA expression during early and persistent Helicobacter pylori infection in nonhuman primates

Harris P.R.; Smythies L.E.; Smith, P. D.; Dubois, A

Keywords: system, animals, infections, expression, cytokine, transcription, monocytes, gene, chain, histopathology, tumor, macrophages, male, polymerase, tissue, gastritis, necrosis, rna, factor-alpha, immune, mucosa, primate, monkey, interleukin-6, article, interleukin-1, factor, controlled, animal, helicobacter, pylori, reverse, gastric, study, response, interleukin, priority, Reaction, nonhuman, journal, Macaca, mulatta, RNA,, Messenger, rhesus, 6, 1beta, Transcriptase, phagocyte

Abstract

The role of mononuclear phagocytes in orchestrating the host responses to Helicobacter pylori is inadequately understood. Therefore, gene expression for the monocyte/macrophage-derived cytokines interleukin (IL)- 1?, IL-6, and tumor necrosis factor (TNF)-? was determined before and during H. pylori infection of rhesus monkeys by use of a highly sensitive quantitative reverse transcriptase polymerase chain reaction. The numbers of molecules of IL-1?, IL-6, and TNF-? mRNA in gastric tissue during early infection (7 weeks) significantly exceeded the preinfection numbers (P < .03). Moreover, the numbers of IL-1?, IL-6, and TNF? mRNA molecules in persistently infected animals (6 years) also were elevated compared with preinfection numbers (P < .02, P = .03, P = .16, respectively). Cytokine gene expression coincided with progressive H. pylori gastritis, confirmed by increased gastritis scores over preinfection scores (P < .005). These findings provide quantitative evidence that H. pylori induces local gene expression of monocyte/macrophage-derived inflammatory cytokines and evokes an innate response in gastric tissue of nonhuman primates.

Más información

Título según SCOPUS: Inflammatory cytokine mRNA expression during early and persistent Helicobacter pylori infection in nonhuman primates
Título de la Revista: JOURNAL OF INFECTIOUS DISEASES
Volumen: 181
Número: 2
Editorial: OXFORD UNIV PRESS INC
Fecha de publicación: 2000
Página de inicio: 783
Página final: 786
Idioma: English
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-0034088755&partnerID=q2rCbXpz
DOI:

10.1086/315257

Notas: SCOPUS