Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-CIC-3 antibody

Duan, D; Zhong, J; Hermoso, M; Satterwhite C.M.; Rossow C.F.; Hatton W.J.; Yamboliev I.; Horowitz B.; Hume J.R.

Keywords: muscle, transport, mouse, heart, fiber, animals, expression, antibody, cells, antigen, ion, protein, cell, chloride, channel, antibodies, mechanism, physiology, mice, laevis, channels, artery, injection, strain, xenopus, oocytes, female, anions, carrier, dialysis, article, myocardium, anion, pig, dog, smooth, pulmonary, oocyte, type, controlled, injections, vascular, animal, current, study, priority, nonhuman, journal, Muscle,, Smooth,, polyclonal, Guinea, 3t3, regulatory

Abstract

1. Intracellular dialysis of NIH/3T3 cells with a commercially available anti-ClC-3 polyclonal antibody (Ab) for ?30 min completely inhibited expressed guinea-pig ClC-3 currents (IgpClC-3), while intracellular dialysis with antigen-preabsorbed anti-ClC-3 Ab failed to affect IgpClC-3. 2. Anti-ClC-3 Ab was used as a selective probe to examine the relationship between endogenous ClC-3 expression and native volume-sensitive outwardly rectifying anion channels (VSOACs) in guinea-pig cardiac cells, canine pulmonary arterial smooth muscle cells (PASMCs) and Xenopus laevis oocytes. Intracellular dialysis or injection of anti-ClC-3 Ab abolished native VSOAC function in cardiac cells and PASMCs and significantly reduced VSOACs in oocytes. In contrast, native VSOAC function was unaltered by antigen-preabsorbed anti-ClC-3 Ab. 3. It is suggested that endogenous ClC-3 represents a major molecular entity responsible for native VSOACs in cardiac and smooth muscle cells and Xenopus oocytes. Anti-CIC-3 Ab should be a useful experimental tool to directly test the relationship between endogenous ClC-3 expression and native VSOAC function, and help resolve existing controversies related to the regulation and physiological role of native VSOACs in a wide variety of different cells.

Más información

Título según SCOPUS: Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-CIC-3 antibody
Título de la Revista: JOURNAL OF PHYSIOLOGY-LONDON
Volumen: 531
Número: 2
Editorial: Wiley
Fecha de publicación: 2001
Página de inicio: 437
Página final: 444
Idioma: English
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-0035281606&partnerID=q2rCbXpz
DOI:

10.1111/j.1469-7793.2001.0437i.x

Notas: SCOPUS