In situ nuclear organization of regulatory machinery

Pockwinse S.M.; Zaidi S.K.; Medina R.F.; Bakshi, R; Kota K.P.; Ali S.A.; Nickerson J.A.; van Wijnen A.J.; Lian J.B.; Stein J.L.; Stein G.S.; Young D.W.; Javed, A; Montecino, M.

Keywords: fluorescence, animals, expression, culture, cell, gene, ultrastructure, nucleus, recovery, metabolism, microscopy, humans, chromosomes, human, regulation, methodology, intermediate, filaments, chromosome, cycle, article, filament, techniques, animal, technique, Cells,, Cultured, Microscopy,, Photobleaching, after

Abstract

Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy. © 2008 Humana Press, a part of Springer Science+Business Media, LLC.

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Título según SCOPUS: In situ nuclear organization of regulatory machinery
Título de la Revista: NEISSERIA MENINGITIDIS
Volumen: 455
Editorial: Humana Press, Inc.
Fecha de publicación: 2008
Página de inicio: 239
Página final: 259
Idioma: eng
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-45549107608&partnerID=q2rCbXpz
DOI:

10.1007/978-1-59745-104-8-17

Notas: SCOPUS