Melatonin reduces cortisol response to ACTH in humans La melatonina reduce la respuesta de cortisol al ACTH en humanos

Campino C.; Arteaga, E; Valenzuela F.; Torres-Farfan, C; Seron-Ferre, M; Trucco, C; Velasco, A; Guzman, S

Keywords: biosynthesis, hormone, transcription, trial, double-blind, chain, gland, secretion, dexamethasone, humans, crossover, immunoassay, human, male, receptor, polymerase, time, melatonin, glands, adult, rna, glucocorticoids, drug, article, blind, young, hydrocortisone, cross-over, method, controlled, glucocorticoid, clinical, reverse, factors, studies, study, 1, adrenal, Reaction, double, RNA,, 2, effect, Receptor,, Messenger, procedure, randomized, Transcriptase, corticotropin, Adrenocorticotropic, Melatonin,, MT2, Mt1

Abstract

Background: Melatonin receptors are widely distributed in human tissues but they have not been reported in human adrenal gland. Aim: To assess if the human adrenal gland expresses melatonin receptors and if melatonin affects cortisol response to ACTH in dexamethasone suppressed volunteers. Material and methods: Adrenal glands were obtained from 4 patients undergoing unilateral nephrectomy-adrenalectomy for renal cancer. Expression of mRNA MT1 and MT2 melatonin receptors was measured by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). The effect of melatonin on the response to intravenous (i.v.) ACTH was tested (randomized cross-over, double-blind, placebo-controlled trial) in eight young healthy males pretreated with dexamethasone (1 mg) at 23:00 h. On the next day, at 08:00 h, an i.v. line was inserted, at 08:30 h, and after a blood sample, subjects ingested 6 mg melatonin or placebo. At 09:00 h, 1-24 ACTH (Cortrosyn, 1 μg/1.73 m2 body surface area) was injected, drawing samples at 0, 15, 30, 45 and 60 minutes after. Melatonin, cortisol, cortisone, progesterone, aldosterone, DHEA-S, testosterone and prolactin were measured by immunoassay. Results: The four adrenal glands expressed only MT1 receptor mRNA. Melatonin ingestion reduced the cortisol response to ACTH from 14.6±1.45 μg/dl at 60 min in the placebo group to 10.8±1.2 μg/dl in the melatonin group (p <0.01 mixed model test). It did not affect other steroid hormone levels and abolished the morning physiological decline of prolactin. Conclusions: The expression of MT1 melatonin receptor in the human adrenal, and the melatonin reduction of ACTH-stimulated cortisol production suggest a direct melatonin action on the adrenal gland (Rev Méd Chile 2008; 136: 1390-7).

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Título según SCOPUS: Melatonin reduces cortisol response to ACTH in humans La melatonina reduce la respuesta de cortisol al ACTH en humanos
Título de la Revista: REVISTA MEDICA DE CHILE
Volumen: 136
Número: 11
Editorial: SOC MEDICA SANTIAGO
Fecha de publicación: 2008
Página de inicio: 1390
Página final: 1397
Idioma: eng
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-62849102055&partnerID=q2rCbXpz
Notas: SCOPUS