Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales

Nuñez, Harold; Ulloa, María Teresa; Guerra, Fabiola; OSORIO, CARLOS G

Abstract

Background: Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative. Aim: To assess if environmental strains lack the full pathogenicity island or if only the tdh gene is deleted. Material and methods: Thirty eight clinical and 66 environmental strains of Vibrio parahaemolyticus were studied. PAI was characterized by polymerase chain reaction (PCR). The presence of tdhA and tdhS genes, was determined by Southern blot. Results: Fifty three environmental strains (80%) lacked a full PAI when compared with clinical strains. In environmental strains, Southern blot and sequence analysis showed that a genetic regi

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Título según SCIELO: Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas cl�nicas y ambientales
Título de la Revista: REVISTA MEDICA DE CHILE
Volumen: 137
Número: 2
Editorial: SOC MEDICA SANTIAGO
Fecha de publicación: 2009
Página de inicio: 208
Página final: 214
Idioma: es
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000200004&lng=en&nrm=iso&tlng=en
DOI:

10.4067/S0034-98872009000200004

Notas: SCIELO