A Functional N-terminal Domain in C/EBP beta-LAP* is Required for Interacting with SWI/SNF and to Repress Ric-8B Gene Transcription in Osteoblasts

Aguilar R.; Grandy, R; Meza D.; Sepulveda, H; Pihan, P; van Wijnen, AJ; Lian, JB; Stein, GS; Stein, JL; Montecino, M.

Abstract

The chromatin remodeling complex SWI/SNF and the transcription factor C/EBP beta play critical roles in osteoblastic cells as they jointly control transcription of a number of bone-related target genes. The largest C/EBP beta isoform, LAP*, possesses a short additional N-terminal domain that has been proposed to mediate the interaction of this factor with SWI/SNF in myeloid cells. Here we examine the requirement of a functional N-terminus in C/EBP beta-LAP* for binding SWI/SNF and for recruiting this complex to the Ric-8B gene to mediate transcriptional repression. We find that both C/EBP beta-LAP* and SWI/SNF simultaneously bind to the Ric-8B promoter in differentiating osteoblasts that repress Ric-8B expression. This decreased expression of Ric-8B is not accompanied by significant changes in histone acetylation at the Ric-8B gene promoter sequence. A single aminoacid change at the C/EBP beta-LAP* N-terminus (R3L) that inhibits C/EBP beta-LAP*-SWI/SNF interaction, also prevents SWI/SNF recruitment to the Ric-8B promoter as well as C/EBP beta-LAP*-dependent repression of the Ric-8B gene. Inducible expression of the C/EBP beta-LAP* R3L protein in stably transfected osteoblastic cells demonstrates that this mutant protein binds to C/EBP beta-LAP*-target promoters and competes with the endogenous C/EBP beta factor. Together our results indicate that a functional N-terminus in C/EBP beta-LAP* is required for interacting with SWI/SNF and for Ric-8B gene repression in osteoblasts. (C) 2014 Wiley Periodicals, Inc.

Más información

Título según WOS: A Functional N-terminal Domain in C/EBP beta-LAP* is Required for Interacting with SWI/SNF and to Repress Ric-8B Gene Transcription in Osteoblasts
Título según SCOPUS: A Functional N-terminal Domain in C/EBP?-LAP* is Required for Interacting with SWI/SNF and to Repress Ric-8B Gene Transcription in Osteoblasts
Título de la Revista: JOURNAL OF CELLULAR PHYSIOLOGY
Volumen: 229
Número: 10
Editorial: WILEY-BLACKWELL
Fecha de publicación: 2014
Página de inicio: 1521
Página final: 1528
Idioma: English
DOI:

10.1002/jcp.24595

Notas: ISI, SCOPUS