Serotype-associated polymorphisms in a partial rpoB gene sequence of Salmonella enterica

Fresno M.; Barreto, M; Gutierrez S. ; Dougnac, C; Abalos P.; Retamal P.

Abstract

Salmonella enterica is a zoonotic bacterium with more than 2500 serotypes, which affect a wide range of hosts and produce diverse clinical outcomes. Strain identification usually involves costly and time-demanding procedures. This paper describes the sequencing of a rpoB hypervariable gene segment (847 bp) that allows identification of serotypes in S. enterica strains isolated from several hosts. The nucleotide similarity values among S. enterica serotypes ranged from 98.23% to 99.88%, with potential usefulness for devising a simple one-step sequencing as a first approach for identification of S. enterica strains. In conclusion, the analysis of polymorphisms in the partial rpoB sequence can discriminate S. enterica strains at the subspecies level.

Más información

Título según WOS: Serotype-associated polymorphisms in a partial rpoB gene sequence of Salmonella enterica
Título de la Revista: CANADIAN JOURNAL OF MICROBIOLOGY
Volumen: 60
Número: 3
Editorial: CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
Fecha de publicación: 2014
Página de inicio: 177
Página final: 181
Idioma: English
DOI:

10.1139/cjm-2013-0872

Notas: ISI