Sex-dependent transcriptome analysis and single nucleotide polymorphism (SNP) discovery in the brine shrimp Artemia franciscana

Valenzuela?Miranda, D; Gallardo-Escarate, C; Valenzuela-Munoz, V; Farlora, R; Gajardo, G.

Keywords: Artemia; Brine shrimp; RNA, Seq; SNP; Transcriptome

Abstract

In order to enhance genomic resources for the brine shrimp Artemia franciscana, RNA-Seq analysis was conducted for adult females and males. Through de novo assembly, 36,896 high quality contigs were obtained, of which 13,749 sequences were annotated with arthropod sequences. Just 4.5% matched against previously reported sequences for Artemia spp. Additionally, different transcriptional patterns between males and females were found, evidencing sex-related transcriptional responses. Furthermore, 221 and 534 putative SNPs were identified exclusively in males and females, respectively. These results will build the foundation for further genomic studies in A. franciscana. (c) 2014 Elsevier B.V. All rights reserved.

Más información

Título según WOS: Sex-dependent transcriptome analysis and single nucleotide polymorphism (SNP) discovery in the brine shrimp Artemia franciscana
Título según SCOPUS: Sex-dependent transcriptome analysis and single nucleotide polymorphism (SNP) discovery in the brine shrimp Artemia franciscana
Título de la Revista: MARINE GENOMICS
Volumen: 18
Número: PB
Editorial: ELSEVIER SCIENCE BV
Fecha de publicación: 2014
Página de inicio: 151
Página final: 154
Idioma: English
DOI:

10.1016/j.margen.2014.10.007

Notas: ISI, SCOPUS