Development and characterization of thirty-three microsatellite markers for the Patagonian sprat, Sprattus fuegensis (Jenyns, 1842), using paired-end Illumina shotgun sequencing
Keywords: ssr, microsatellite, pcr primers, Sprattus, Illumina, PAL_FINDER
Abstract
We isolated and characterized a total of 33 microsatellite loci from the Patagonian sprat Sprattus fuegensis, a recent exploited marine resource with a conservation status unknowing. Loci were screened in 24 individuals from the inshore waters of the Aysen Fjord, Chile. The number of alleles per locus ranged from 7 to 24, observed heterozygosity ranged from 0.217 to 0.875, and the probability of identity values ranged from 0.006 to 0.133. These new loci will provide tools for examining population genetic structure, estimating effective population size and provide information to fisheries management and conservation.
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Título según WOS: | Development and characterization of thirty-three microsatellite markers for the Patagonian sprat, Sprattus fuegensis (Jenyns, 1842), using paired-end Illumina shotgun sequencing |
Título según SCOPUS: | Development and characterization of thirty-three microsatellite markers for the Patagonian sprat, Sprattus fuegensis (Jenyns, 1842), using paired-end Illumina shotgun sequencing |
Título de la Revista: | CONSERVATION GENETICS RESOURCES |
Volumen: | 6 |
Número: | 4 |
Editorial: | Springer |
Fecha de publicación: | 2014 |
Página de inicio: | 833 |
Página final: | 836 |
Idioma: | English |
DOI: |
10.1007/s12686-014-0281-x |
Notas: | ISI, SCOPUS |