Eficacia del diagnóstico diferencial de tuberculosis mediante reacción de polimerasa en cadena en lesiones granulomatosas de tejidos fijados en formalina e incluidos en parafina

Sonia Montenegro; carolina delgado; Susana Pineda; Cristian Reyes; Tiare de la Barra; Claudia Cabezas; Loreto Spencer; Francisco Mucientes

Abstract

Background: Granulomatous lesions occur in tuberculosis (TB), other infections, toxic, allergic, and autoimmune diseases among others. In absence of a an acid-fast bacilli (AFB) confirmation of TB is necessary. Objective: To assess the efficacy of PCR for TB detection and to correlate with granuloma histology and AFB staining. Methods: We analyzed 380 fixed paraffin-embedded tissues (PETs) of granulomas with and without caseous necrosis; suppurative; sarcoidal; or of chronic nonspecific nature. Nested PCR-IS6110 for Mycobacterium tuberculosis complex (MTB) and a nested pan-Mycobacterium for the hsp65 gene were used for Mycobacterium spp detection. Results: PCR was more sensitive than AFB staining for all five catageories of granulomas: G1: PCR 71%, AFB staining 28%. G2: PCR 37%, AFB 8%. G3: PCR 17%, AFB staining 7%. G4: PCR 8%, AFB staining 4%. G5: PCR 6%, AFB staining 0%. Conclusions: Molecular diagnosis of TB using PCR-based testing is a fast, efficacious and sensitive method that increased the accuracy of PET histological diagnosis associated with granulomatous lesions.

Más información

Título según SCIELO: Eficacia del diagn�stico diferencial de tuberculosis mediante reacci�n de polimerasa en cadena en lesiones granulomatosas de tejidos fijados en formalina e incluidos en parafina
Título de la Revista: REVISTA CHILENA DE INFECTOLOGIA
Volumen: 31
Número: 6
Editorial: SOC CHILENA INFECTOLOGIA
Fecha de publicación: 2014
Página de inicio: 676
Página final: 681
DOI:

10.4067/S0716-10182014000600006

Notas: SCIELO