Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using the Endomembrane Trafficking Network

Rodriguez-Furlan, C; Hicks, GR; Norambuena, L

Keywords: endocytosis, exocytosis, screening, Chemical genomics, Organelle markers, Multidrug treatments

Abstract

The plant endomembrane trafficking system is a highly complex set of processes. This complexity presents a challenge for its study. Classical plant genetics often struggles with loss-of-function lethality and gene redundancy. Chemical genomics allows overcoming many of these issues by using small molecules of natural or synthetic origin to inhibit specific trafficking proteins thereby affecting the processes in a tunable and reversible manner. Bioactive chemicals identified by high-throughput phenotype screens must be characterized in detail starting with understanding of the specific trafficking pathways affected. Here, we describe approaches to characterize bioactive compounds that perturb vesicle trafficking. This should equip researchers with practical knowledge on how to identify endomembrane-specific trafficking pathways that may be perturbed by specific compounds and will help to eventually identify molecular targets for these small molecules.

Más información

Título según WOS: Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using the Endomembrane Trafficking Network
Título según SCOPUS: Chemical genomics: Characterizing target pathways for bioactive compounds using the endomembrane trafficking network
Título de la Revista: NEISSERIA MENINGITIDIS
Volumen: 1174
Editorial: Humana Press, Inc.
Fecha de publicación: 2014
Página de inicio: 317
Página final: 328
Idioma: English
DOI:

10.1007/978-1-4939-0944-5_22

Notas: ISI, SCOPUS