Neurite outgrowth in developing mouse spinal cord neurons is modulated by glycine receptors

Tapia, JC; Cárdenas AM; Nualart, F.; Mentis, GZ; Navarrete R.; Aguayo, LG

Abstract

The effect of glycine receptor activation on neurite outgrowth and survival was studied in 5 DIV (days in vitro) spinal neurons. These neurons were depolarized by spontaneous synaptic activity and by glycine, but not by glutamate. These responses were accompanied by increases in intracellular calcium concentration measured with Indo-1 and Fluo-3. Glycine (100 mu M, 48 h) increased (46 +/- 6%) the number of primary neurites and total neuritic length. This effect was mediated by synaptic activity and calcium influx because TTX (1 mu M) and nimodipine (4 mu M) blocked the stimulatory effect of glycine. Neuronal survival, on the other hand, was not affected. This study shows for the first time the modulatory effect of glycine receptors on spinal neuron development. NeuroReport 11:3007-3010 (C) 2000 Lippincott Williams & Wilkins.

Más información

Título según WOS: Neurite outgrowth in developing mouse spinal cord neurons is modulated by glycine receptors
Título según SCOPUS: Neurite outgrowth in developing mouse spinal cord neurons is modulated by glycine receptors
Título de la Revista: NEUROREPORT
Volumen: 11
Número: 13
Editorial: LIPPINCOTT WILLIAMS & WILKINS
Fecha de publicación: 2000
Página de inicio: 3007
Página final: 3010
Idioma: English
URL: http://content.wkhealth.com/linkback/openurl?sid=WKPTLP:landingpage&an=00001756-200009110-00036
DOI:

10.1097/00001756-200009110-00036

Notas: ISI, SCOPUS