Glycoprotein Ib/IX complex is the target in rifampicin-induced immune thrombocytopenia

Pereira J.; Hidalgo, P.; Ocqueteau, M; Blacutt, M; Marchesse, M; Nien, Y; Letelier, L.; Mezzano D.

Abstract

Thrombocytopenia is a major adverse effect of several drug treatments. Rifampicin has been recognized as a cause of immune thrombocytopenia during intermittent high-dose therapy. We characterized the antibody of a patient who presented with purpura and thrombocytopenia during treatment of tuberculosis with rifampicin. Drug-dependent binding of the antibody to platelets was demonstrated by flow cytometry. In a glycoprotein-specific immunoassay, the binding epitope of the IgG antibody was found in the glycoprotein Ib/IX complex, using four different monoclonal antibodies (mAbs) against various epitopes on the GPIb/IX complex, as well as mAbs against GPIIb/IIIa, GPIa/IIa and GPIV. By immunoprecipitation of biotin-labelled platelets, reactivity of the antibody with GPIb/IX was found only in the presence of the drug. These findings clearly demonstrate that rifampicin induces the formation of drug-dependent antibodies capable of causing thrombocytopenia. The binding site of the rifampicin-dependent antibody, located in the GPIb/IX complex, seems to be a favoured target for antibodies induced by different drugs.

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Título según WOS: Glycoprotein Ib/IX complex is the target in rifampicin-induced immune thrombocytopenia
Título según SCOPUS: Glycoprotein Ib/IX complex is the target in rifampicin-induced immune thrombocytopenia
Título de la Revista: BRITISH JOURNAL OF HAEMATOLOGY
Volumen: 110
Número: 4
Editorial: Wiley
Fecha de publicación: 2000
Página de inicio: 907
Página final: 910
Idioma: English
URL: http://doi.wiley.com/10.1046/j.1365-2141.2000.02299.x
DOI:

10.1046/j.1365-2141.2000.02299.x

Notas: ISI, SCOPUS