Proliferation and osmotic tolerance of renal inner medullary epithelial cells in vivo and in cell culture

Zhang Z.; Cai Q.; Michea, L.; Dmitrieva, NI; Andrews P.; Burg, MB

Abstract

Renal inner medullary (IM) cells survive interstitial osmolality that ranges from 600 to 1,700 mosmol/kgH2O or more. In contrast, much smaller acute changes killed the cells previously studied in tissue culture, such as mouse IM collecting duct 3 (mIMCD3) cells, that are immortalized with SV40 and proliferate rapidly. Proliferation and DNA replication sensitize mIMCD3 cells to hypertonicity. In the present studies, we observed that proliferating cells were scarce in rat IM. Then, we prepared passage 2 mouse IM epithelial (p2mIME) cells. They have a much lower incidence of DNA replication than do mIMCD3 cells. p2mIME cells survive much greater acute increases in NaCl than do mIMCD3 cells and also tolerate significantly greater acute increases of urea and of NaCl plus urea, but still not to levels as high as occur in vivo. We conclude that immortalization and continued DNA replication account for part of the previously observed difference in osmotic tolerance between IM cells in vivo and in cell culture but that other factors must also be involved.

Más información

Título según WOS: Proliferation and osmotic tolerance of renal inner medullary epithelial cells in vivo and in cell culture
Título según SCOPUS: Proliferation and osmotic tolerance of renal inner medullary epithelial cells in vivo and in cell culture
Título de la Revista: AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
Volumen: 283
Número: 2
Editorial: AMER PHYSIOLOGICAL SOC
Fecha de publicación: 2002
Página de inicio: F302
Página final: F308
Idioma: English
DOI:

10.1152/ajprenal.00038.2002

Notas: ISI, SCOPUS