A rapid, sensitive and inexpensive method for detection of grapevine red blotch virus without tissue extraction using loop-mediated isothermal amplification

Romero Romero J.L.; Carver G.D.; Arce Johnson P.; Perry K.L.; Thompson J.R.

Abstract

Grapevine red blotch virus (GRBV) is an emerging virus of significant viticultural importance throughout North America. Here, we report the development of a simple protocol for point-of-use detection of GRBV. Extraction of nucleic acids is not required; instead, the whole intact plant can simply be pricked with a sterile pipette tip, which is then incubated in sterile distilled water to provide the sample template in a loop-mediated isothermal amplification (LAMP) reaction. This method is 10,000 times more sensitive than conventional PCR, costs under a dollar per sample, and can be completed from sampling to readout in just over half an hour.

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Título según WOS: A rapid, sensitive and inexpensive method for detection of grapevine red blotch virus without tissue extraction using loop-mediated isothermal amplification
Título según SCOPUS: A rapid, sensitive and inexpensive method for detection of grapevine red blotch virus without tissue extraction using loop-mediated isothermal amplification
Título de la Revista: ARCHIVES OF VIROLOGY
Volumen: 164
Número: 5
Editorial: SPRINGER WIEN
Fecha de publicación: 2019
Página de inicio: 1453
Página final: 1457
Idioma: English
DOI:

10.1007/s00705-019-04207-y

Notas: ISI, SCOPUS