Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors

Krüger, Kerstin; Fiore, Nicola

Keywords: traps, survey, sampling strategies, leafhoppers, Psyllids, Planthoppers, Vacuum sampling, Potential vectors of phytoplasmas, Transmission trials

Abstract

To reduce the spread of phytoplasmas in a crop or in a certain geographic area, epidemiological studies are of crucial importance in determining which insect species transmit these pathogens. In this chapter, we describe methods of capturing the insect vectors of phytoplasmas and the criteria for choosing the method(s) according to the objective to be achieved.

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Fecha de publicación: 2019
Página de inicio: 37
Página final: 52
Idioma: English
Financiamiento/Sponsor: Springer Science + Business Media
URL: https://www.springer.com/us/book/9781493988365
Notas: This book presents a set of modern protocols forming a solid background for who want to start or improve research programme on phytoplasmas. Chapters guide readers through detailed techniques for maintaining phytoplasma collections, border inspection, detection of different phytoplasma strains, new pipelines to produce phytoplasma genome draft, protocols for phytoplasma gene expression analyses, and methods for the investigation of the phloem tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.