High-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile

Anabalón, Leonardo; Encina?Montoya, Francisco; Sánchez, Pamela; Solano, Jaime; Benavente, Felipe; Guiñez, Basilio; Olivares, Flavio; Oberti, Carlos; Vega, Rolando

Abstract

Easy, economic, precise species authentication is currently necessary in many areas of research and diagnosis in molecular biology applied to conservation studies of endangered species. Here, we present a new method for the identification of three fox species of the Lycalopex genus in Chile. We developed an assay based on high-resolution melt analysis of the mitochondrial cytochrome B gene, allowing a simple, low cost, fast, and accurate species determination. To validate the assay applicability for noninvasive samples, we collected fecal samples in the Atacama Desert, finding unexpectedly one species outside of its known distribution range. We conclude that the assay has a potential to become a valuable tool for a standardized genetic monitoring of the Lycalopex species in Chile.

Más información

Título según WOS: High-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile
Título según SCOPUS: High-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile
Título de la Revista: ECOLOGY AND EVOLUTION
Volumen: 9
Número: 13
Editorial: Wiley
Fecha de publicación: 2019
Página de inicio: 7448
Página final: 7454
Idioma: English
DOI:

10.1002/ece3.5230

Notas: ISI, SCOPUS