Enantiomeric separation of D- and L-carnitine by integrating on-line derivatization with capillary zone electrophoresis

Mardones, C; Rios, A; Valcarcel, M; Cicciarelli, R

Abstract

A new capillary zone electrophoretic method has been developed for the enantiomeric separation and quantification of enantiomers of carnitine. D- and L-carnitine were derivatized with 9-fluorenylmethyl chloroformate in a flow system, working on-line with the capillary electrophoretic equipment. The separation was performed using a selective chiral buffer containing 2,6-dimethyl-beta-cyclodextrin (heptakis). Triethanolamine was used as electroosmotic modifier and the separation was carried out in a uncoated capillary. Under the optimal conditions the resolution between D- and L-carnitine was 1.2 and the limits of detection for both isomers were about 5.0 mu M. The proposed method was applied to the determination of D-carnitine in excess of L-carnitine in synthetic samples, and the results demonstrated that the maximal D-:L-carnitine ratio determined was approximate to 1:100. (C) 1999 Elsevier Science B.V. All rights reserved.

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Título según WOS: ID WOS:000081668400028 Not found in local WOS DB
Título de la Revista: JOURNAL OF CHROMATOGRAPHY A
Volumen: 849
Número: 2
Editorial: Elsevier
Fecha de publicación: 1999
Página de inicio: 609
Página final: 616
DOI:

10.1016/S0021-9673(99)00602-0

Notas: ISI