Abstract

The skin interacts permanently with the environment and upon trauma, it is expected that wound heals properly. This healing process is complex because involves several cell types working harmoniously to end with a functional scarring. At this regard, extracellular collagen is crucial to promote wound closing. Under excessive extracellular glucose, not only collagen undergoes direct glycation but also, fibroblast are subjected to oxidative stress. A cellular condition that affects the quality of the collagen synthesized by dermal fibroblasts, which in turn supports the keratinocytes at the epidermis. Our goal was to compare the effects of normo and high extracellular glucose on the ability of human dermal fibroblasts to generate collagen, determining its abundance and functionality. By studying fibroblast and keratinocytes functionality, we expect to have an in vitro platform to evaluate the efficiency of treatments to improve wound healing in pathological scenarios like chronic non-compensated diabetes.