Abstract

Phenolic compounds in oilseeds occur in the free, esterified and insoluble-bound forms and serve as natural antioxidants by preventing food oxidation as well as oxidative stress and various disorders in the body. In this work, free, esterified and insoluble-bound phenolics were extracted from defatted camelina and sophia seeds. Samples were evaluated for their total contents of phenolics (TPC), flavonoids (TFC) and proanthocyanidins (PC) as well as the antioxidant activity of their various phenolic fractions. Several in vitro methods, namely Trolox equivalent antioxidant capacity (TEAC), reducing power (RP) and metal chelation activity were used for all fractions. High performance liquid chromatography-diode array detection-tandem mass spectrometry (HPLCDAD-MS/MS) identified 29 phenolics belonging to phenolic acids and their derivatives, flavonoids and procyanidins in different fractions of camelina and sophia extracts. Esterified fraction was the predominant form of phenolics in both seeds. Thus, camelina and sophia seeds may serve as viable functional food ingredients with protective antioxidant potential.