Human brain synembryn interacts with Gs alpha and Gq alpha and is translocated to the plasma membrane in response to isoproterenol and carbachol

Klattenhoff, C; Montecino, M.; Soto, X; Guzman, L; Romo, X.; Garcia, MD; Mellstrom, B; Naranjo, JR; Hinrichs, MV; Olate, J

Abstract

Heterotrimeric G-proteins transduce signals from heptahelical transmembrane receptors to different effector systems, regulating diverse complex intracellular pathways and functions. In brain, facilitation of depolarization-induced neurotransmitter release for synaptic transmission is mediated by Gs? and Gq?. To identify effectors for G?-proteins, we performed a yeast two-hybrid screening of a human brain cDNA library, using the human G?s protein as a bait. We identified a protein member of the synembryn family as one of the interacting proteins. Extending the study to other G? subunits, we found that Gq? also interacts with synembryn, and these interactions were confirmed by in vitro pull down studies and by in vivo confocal laser microscopy analysis. Furthermore, synembryn was shown to translocate to the plasma membrane in response to carbachol and isoproterenol. This study supports recent findings in C. elegans where, through genetic studies, synembryn was shown to act together with Gq? regulating neuronal transmitter release. Based on these observations, we propose that synembryn is playing a similar role in human neuronal cells. © 2003 Wiley-Liss, Inc.

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Título según WOS: Human brain synembryn interacts with Gs alpha and Gq alpha and is translocated to the plasma membrane in response to isoproterenol and carbachol
Título según SCOPUS: Human brain synembryn interacts with Gs? and Gq? and is translocated to the plasma membrane in response to isoproterenol and carbachol
Título de la Revista: JOURNAL OF CELLULAR PHYSIOLOGY
Volumen: 195
Número: 2
Editorial: Wiley
Fecha de publicación: 2003
Página de inicio: 151
Página final: 157
Idioma: English
URL: http://doi.wiley.com/10.1002/jcp.10300
DOI:

10.1002/jcp.10300

Notas: ISI, SCOPUS