Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile

Cerda C.; Veloso-Frías J.; Lobos-Chávez F.; Oyarzún-Ruiz P.; Henríquez A.; Loyola M.; Silva-de la Fuente M.C.; Ortega R.; Letelier R.; Landaeta-Aqueveque C.

Abstract

This research aimed to determine the presence of paramphistomids in cattle slaughtered in a slaughterhouse of the Ruble Region of Chile, to identify flukes and to analyze the frequency of these parasites in the Maule, Nuble, and Biobio administrative regions of Chile. Between October of 2016 and April of 2017, rumens of 494 cattle were examined for flukes in the forestomachs. Worms were identified morphologically and, in addition, molecular analysis of the internal transcriber spacer region 2 of the fluke's DNA was done and phylogenetic analyses were performed with Bayesian inference in 14 worms. The frequency was analyzed by locality (low- or highlands) and age. The overall frequency was 11.24%. The district with the highest frequency of presentation was Chihli Viejo (30.8%). Districts in the lowlands had similar frequencies to those in the mountain lands (p=0.1). The frequency of flukes was significantly higher in adult animals than in young ones (p<0.01). We obtained a 460 bp-length fragment of DNA that was identical to the sequences previously identified as Paramphistomum cervi and Calicophoron microbothrioides, and performed morphological analyses confirmed that our samples belonged to C. microbothrioides. This is the first published study of C. microbothrioides in Chile.

Más información

Título según WOS: Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile
Título según SCOPUS: Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile [Identificação morfológica e molecular com análise de frequência de Calicophoron microbothrioides na região central do Chile]
Título de la Revista: REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
Volumen: 28
Número: 4
Editorial: BRAZILIAN COLL VETERINARY PARASITOLOGY
Fecha de publicación: 2019
Página de inicio: 582
Página final: 591
Idioma: English
DOI:

10.1590/S1984-29612019076

Notas: ISI, SCOPUS