Cilium-attached and excised patch-clamp recordings of odourant-activated Ca-dependent K channels from chemosensory cilia of olfactory receptor neurons

Delgado, R; Bacigalupo, J

Abstract

It has previously been proposed that a Ca2+-dependent K + conductance is implicated in the inhibitory odourant response in rat and toad olfactory receptor neurons. Previous whole-cell and single-channel measurements on inside-out excised patches, in addition to immunochemical evidence, indicated the presence of Ca2+-dependent K+ channels in olfactory cilia, the transducing structures of these sensory cells. Ca2+-dependent K+ channels opened in 'on-cilium' membrane patches from C. caudiverbera upon odourant stimulation. Furthermore, after excision in the inside-out configuration, the channel could be opened by micromolar Ca2+, in a Ca2+-dependent fashion, but it was unresponsive to cyclic AMP. We estimated that the Ca2+ concentration in the proximity of a Ca2+-dependent K+ channel within the cilia reaches at least 100 ?? during the odour response. The K + channel displayed a higher selectivity for K+ than for Na+. Our results support a role for this Ca2+-dependent K+ channel in chemotransduction.

Más información

Título según WOS: Cilium-attached and excised patch-clamp recordings of odourant-activated Ca-dependent K channels from chemosensory cilia of olfactory receptor neurons
Título según SCOPUS: Cilium-attached and excised patch-clamp recordings of odourant-activated Ca-dependent K channels from chemosensory cilia of olfactory receptor neurons
Título de la Revista: EUROPEAN JOURNAL OF NEUROSCIENCE
Volumen: 20
Número: 11
Editorial: WILEY-BLACKWELL
Fecha de publicación: 2004
Página de inicio: 2975
Página final: 2980
Idioma: English
URL: http://doi.wiley.com/10.1111/j.1460-9568.2004.03778.x
DOI:

10.1111/j.1460-9568.2004.03778.x

Notas: ISI, SCOPUS