First genome sequence of Chilean Brucella canis SCL strain provides insights of the epidemiology and virulence factors explaining differences between geographical origins

Borie, Consuelo; Bravo, Cristian; Dettleff, Phillip; Galarce, Nicolas; Dorner, Jessica; Martinez, Victor A.

Abstract

Background: Brucella canis is the etiological agent of canine brucellosis, a worldwide neglected zoonosis that constitutes one of the major infectious causes of infertility and reproductive failure in dogs. Although genomic information available for this pathogen has increased in recent years, here we report the first genome sequencing of a B. canis strain in Chile, and the differences in virulence genes with other B. canis strains. Results: Genome assembly produced a total length of 3,289,216 bp, N50 of 95,163 and GC% of 57.27, organized in 54 contigs in chromosome I, and 21 contigs in chromosome II. The genome annotation identified a total of 1,981 CDS, 3 rRNA and 36 tRNA in chromosome I, and 1,113 CDS and 10 tRNA in chromosome II. There is little variation between the different strains and the SCL isolate. Phylogenetic analysis showed that the Chilean SCL strain is closely related to B. canis and B. suis strains. Small differences were found when compared to the Serbian isolate, but all strains shared the same recent common ancestor. Finally, changes in the sequence of some virulence factors showed that the SCL strain is similar to other South American B. canis strains. Conclusions: This work sequenced and characterized the complete genome of B. canis SCL strain, evidencing the complete presence of all the genes of the virB operon, and minor changes in outer membrane proteins and in the urease operon. Our data suggest that B. canis was introduced from North America and then spread throughout the South American continent.

Más información

Título de la Revista: Electronic Journal of Biotechnology
Volumen: 49
Editorial: UNIV CATOLICA DE VALPARAISO
Fecha de publicación: 2021
Página de inicio: 1
Página final: 4
URL: https://www.sciencedirect.com/science/article/pii/S0717345820300518
Notas: WOS Core collection ISI