Quantification of Bacteria Residing in Caenorhabditis elegans Intestine

Palominos, M. Fernanda; Calixto, Andrea

Abstract

Quantification of intestinal colonization by pathogenic or commensal bacteria constitute a critical part of the analysis to understand host-microbe interactions during different time points of their interplay. Here we detail a method to isolate non-pathogenic and pathogenic bacteria from C. elegans intestines, and classify gut phenotypes induced by bacterial pathogens using fluorescently-tagged bacteria. Furthermore, these methods can be used to isolate and identify new culturable bacterial species from natural microbiomes of wild nematodes.

Más información

Título de la Revista: BIO-PROTOCOL
Volumen: 10
Número: 9
Editorial: BIO-PROTOCOL
Fecha de publicación: 2020
DOI:

10.21769/BioProtoc.3605