Abstract

In this paper, we present imaging processing advances and applications of mid-wavelength infrared (MWIR) microscopy imaging. Practical issues related to imaging acquisition, image nonuniformity correction, infrared image quality assessment, and even the MWIR microscope optical Point Spread Function experimental estimation are discussed. The built-up MWIR microscope imaging system allows us to analyse thermal features near to the system diffraction limit, up to 200 frames per second and to focus on less than 2 mm2 area. On basis of this technology, our group has been focused efforts in exothermal biological processes, achieving the results exposed in this paper.