Abstract

Fluoroquinolones are antimicrobials approved for use in domestic animals but are not allowed in laying birds because they generate residues in eggs that compromise food safety, although they are usually applied against infectious diseases that defy animal life. This study was performed to establish depletion in the edible compartments of the egg. Laying hens in postural peak were used, divided into two groups, group A (N = 15) and B (N = 10) receiving 7.3 and 1.5 mg / kg of danofloxacin (DFX) and marbofloxacin (MFX) respectively in the drinking water 5for 11 days. At the end of the administration, eggs were collected daily and separated into clear and yolk. In group A the extraction of the analyte was performed with 200 μL of sample, 200 μL of water, 800 μL of a solution of methanol: water: perchloric acid 50: 50: 2 v / v / v and norfloxacin as internal standard and in group B with methanol: water: perchloric acid: phosphoric acid (50: 50: 2 v / v / v) and enrofloxacin as internal standard.The whole was centrifuged 25 minutes at 13500 rpm at 4 ° C. Separation and quantification was performed by HPLC reverse phase isocratic elution with C-18 column, fluorescence detector at lex 295 nm and lem 490 (DFX) and 500 nm (MBX) and mobile phase composed of water, acetonitrile and triethylamine (79: 19:1 v/v/v) at pH 3. Using peak areas of known concentrations, the concentrations of test samples were calculated by simple linear regression. According to the complexity of matrix studied, the procedures applied are simple, fast and sensitive for monitoring programs and control of residues. Levels that declined significantly after the second day were established, and persists until 9 and 15 (DFX) and 8 and 9 days (MBX) in clear and yolk, respectively, Being more relevant in yolk according to the liposolubility of fluoroquinolones and the time required by each egg compartment for its formation, according to the extension of the treatment