Abstract

Cultivation of Agarophyton chilensis, common name "pelillo", in Chile began 40 years ago. Technology adopted has been based on culture by vegetative propagation and culture from spores. Mass seaweed culture is associated with increased instances of pests and/or disease, as has been observed in the agricultural sector. The aim of this work is to study the pest alga Rhizoclonium sp, and its development under different controlled environmental conditions, given that this alga has affected "pelillo" production in southern Chile since 2015. Fresh thalli of the pest alga collected from culture centers located in Maullin river were described morphologically in order to confirm the identity of the species. Filaments were cultured under different temperature (10, 16 and 20 degrees C), photoperiod (8:16; 12:12 and 16:8 L:D), salinity (5; 10; 20; 27 and 30 psu) and nutrient (0.14 and 0.28 mmol/L nitrate) conditions to evaluate growth and reproduction. Nevertheless, given the taxonomic status situation of the polyphyletic genus Rhizoclonium, the pest is named Rhizoclonium-like. It formed lateral branches under culture conditions tested and showed a wide range of tolerance to temperature, salinity, photoperiod and nitrate concentration. Growth increases with temperature, reaching a daily growth rate of between 5.5 and 5.9%/day at 16 and 20 degrees C respectively. Rhizoclonium-like reproduction is described for the first time under culture conditions in Chile. Formation of reproductive structures occurs in the vegetative cells generating reproductive structures (zoosporangia) that mature under 16 degrees C, salinity >20 psu and nitrate concentration >0,14 mmol/L, observing release and germination of spores. After reproduction filaments do not decay and grow again.