Continuous biohydrogen production by a degenerated strain of Clostridium acetobutylicum ATCC 824

Guerrero, Karlo; Gallardo, Roberto; Paredes, Ivan; Quintero, Julian; Mau, Silvia; Conejeros, Raul; Carlos Gentina, Juan; Aroca, German

Abstract

Degenerated strains of Clostridium acetobutylicum lack the ability to produce solvents and to sporulate, allowing the continuous production of hydrogen and organic acids. A degenerated strain of Clostridium acetobutylicum was obtained through successive batch cultures. Its kinetic characterization showed a similar specific growth rate than the wild type (0.25 h(-1)), a higher butyric acid production of 6.8 g-L-1 and no solvents production. A steady state was reached in a continuous culture at a dilution rate of 0.1 h(-1), with a constant hydrogen production of 507 mL.h(-1), corresponding to a volumetric rate of 6.10 L.L(-1)d(-1), and a yield of 2.39 mol of H-2 per mole of glucose which represents 60% of the theoretical maximum yield. These results suggest that the degeneration is an interesting alternative for hydrogen production with this strain, obtaining a high hydrogen production in a continuous culture with cells in a permanent acidogenic state. (C) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.

Más información

Título según WOS: Continuous biohydrogen production by a degenerated strain of Clostridium acetobutylicum ATCC 824
Título de la Revista: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Volumen: 46
Número: 7
Editorial: PERGAMON-ELSEVIER SCIENCE LTD
Fecha de publicación: 2021
Página de inicio: 5100
Página final: 5111
DOI:

10.1016/J.IJHYDENE.2020.11.104

Notas: ISI