Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients

Ortega, Francisco G.; Regiart, Matias D.; Rodriguez-Martinez, Alba; de Miguel-Perez, Diego; Serrano, Maria J.; Lorente, Jose A.; Tortella, Gonzalo; Rubilar, Olga; Sapag, Karim; Bertotti, Mauro; Fernandez-Baldo, Martin A.

Abstract

This study is focused on identifying novel epithelial markers in circulating extracellular vesicles (EVs) through the development of a dual sandwich-type electrochemical paper-based immunosensor for Claudin 7 and CD81 determination, as well as its validation in breast cancer (BC) patients. This immunosensor allows for rapid, sensitive, and label-free detection of these two relevant BC biomarkers. Under optimum conditions, the limit of detection for Claudin 7 was 0.4 pg mL(-1), with a wide linear range of 2 to 1000 pg mL(-)(1), while for CD81, the limit of detection was 3 pg mL(-1), with a wide linear range of 0.01 to 10 ng mL(-1). Finally, we validated Claudin 7 and CD81 determination in EVs from 60 BC patients and 20 healthy volunteers, reporting higher diagnostic accuracy than the one observed with classical diagnostic markers. This analysis provides a low-cost, specific, versatile, and user-friendly strategy as a robust and reliable tool for early BC diagnosis.

Más información

Título según WOS: Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients
Título de la Revista: ANALYTICAL CHEMISTRY
Volumen: 93
Número: 2
Editorial: AMER CHEMICAL SOC
Fecha de publicación: 2021
Página de inicio: 1143
Página final: 1153
DOI:

10.1021/ACS.ANALCHEM.0C04180

Notas: ISI