TRPV1-Estradiol Stereospecific Relationship Underlies Cell Survival in Oxidative Cell Death

Ramirez-Barrantes, Ricardo; Carvajal-Zamorano, Karina; Rodriguez, Belen; Cordova, Claudio; Lozano, Carlo; Simon, Felipe; Diaz, Paula; Munoz, Pablo; Marchant, Ivanny; Latorre, Ramon; Castillo, Karen; Olivero, Pablo

Abstract

17 beta-estradiol is a neuronal survival factor against oxidative stress that triggers its protective effect even in the absence of classical estrogen receptors. The polymodal transient receptor potential vanilloid subtype 1 (TRPV1) channel has been proposed as a steroid receptor implied in tissue protection against oxidative damage. We show here that TRPV1 is sufficient condition for 17 beta-estradiol to enhance metabolic performance in injured cells. Specifically, in TRPV1 expressing cells, the application of 17 beta-estradiol within the first 3 h avoided H2O2-dependent mitochondrial depolarization and the activation of caspase 3/7 protecting against the irreversible damage triggered by H2O2. Furthermore, 17 beta-estradiol potentiates TRPV1 single channel activity associated with an increased open probability. This effect was not observed after the application of 17 alpha-estradiol. We explored the TRPV1-Estrogen relationship also in primary culture of hippocampal-derived neurons and observed that 17 beta-estradiol cell protection against H2O2-induced damage was independent of estrogen receptors pathway activation, membrane started and stereospecific. These results support the role of TRPV1 as a 17 beta-estradiol-activated ionotropic membrane receptor coupling with mitochondrial function and cell survival.

Más información

Título según WOS: TRPV1-Estradiol Stereospecific Relationship Underlies Cell Survival in Oxidative Cell Death
Título de la Revista: FRONTIERS IN PHYSIOLOGY
Volumen: 11
Editorial: FRONTIERS MEDIA SA
Fecha de publicación: 2020
DOI:

10.3389/fphys.2020.00444

Notas: ISI