Abstract

Simple Summary Udder infection by bacteria such as Staphylococcus aureus cause economic losses to dairy production. An effective vaccine is required to control S. aureus mastitis. To develop an effective vaccine, a good experimental infection model is required. Infusion of bacteria into the udder can overwhelm the host because it bypasses physical barriers and defense mechanisms in the teat canal. The objective of this study was to develop Staphylococcus aureus mastitis challenge model that mimics natural infection. Eight Holstein dairy cows within 1st to 3rd parity at early non-milking period were randomly divided into experimental (n = 5) and control (n = 3) groups. All teats of experimental cows were challenged by dipping into S. aureus culture suspension, whereas those of control cows were dipped into phosphate-buffered saline. Bacteria in the mammary secretion was determined by bacteriological culture. The antibody titer in blood was tested by enzyme-linked immunosorbent assay (ELISA). Other analyses, which include somatic cell count, rectal body temperature, inflammatory changes in mammary secretion, and gland tissues, were assessed. Results showed that three and one of five experimental cows developed subclinical and clinical mastitis, respectively. The remaining cow was infected with Staphylococcus chromogenes. In conclusion, experimental S. aureus mastitis can be induced by teat dipping in the bacterial culture.