High-Temperature Stress Effect on the Red Cusk-Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage
Abstract
Environmental stressors, such as temperature, are relevant factors that could generate a negative effect on several tissues in fish. A key fish species for Chilean aquaculture diversification is the red cuskâeel (Genypterus chilensis), a native fish for which knowledge on environmental stressors effects is limited. This study evaluated the effects of highâtemperature stress on the liver of red cuskâeel in control (14 °C) and highâtemperature (19 °C) groups using multiple approaches: determination of plasmatic hepatic enzymes (ALT, AST, and AP), oxidative damage evaluation (AP sites, lipid peroxidation, and carbonylated proteins), and RNAâseq analysis. High-temperature stress generated a significant increase in hepatic enzyme activity in plasma. In the liver, a transcriptional regulation was observed, with 1239 downâregulated and 1339 upâregulated transcripts. Additionally, highâtemperature stress generated oxidative stress in the liver, with oxidative damage and transcriptional modulation of the antioxidant response. Furthermore, an unfolded protein response was observed, with several pathways enriched, as well as a heat shock response, with several heat shock proteins up regulated, suggesting candidate biomarkers (i.e., serpinh1) for thermal stress evaluation in this species. The present study shows that high-temperature stress generated a major effect on the liver of red cuskâeel, knowledge to consider for the aquaculture and fisheries of this species.
Más información
| Título según WOS: | High-Temperature Stress Effect on the Red Cusk-Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage |
| Título según SCOPUS: | HighâTemperature Stress Effect on the Red CuskâEel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage |
| Título de la Revista: | Biology |
| Volumen: | 11 |
| Número: | 7 |
| Editorial: | Multidisciplinary Digital Publishing Institute (MDPI) |
| Fecha de publicación: | 2022 |
| Idioma: | English |
| DOI: |
10.3390/biology11070990 |
| Notas: | ISI, SCOPUS |