Purification and characterization of a p-coumarate decarboxylase and a vinylphenol reductase from Brettanomyces bruxellensis

GODOY, L.; Martínez C; Carrasco N.; GANGA, MA

Abstract

The presence of Brettanomyces bruxellensis has been correlated with an increase of phenolic aromas in wine. The production of these aromas results from the metabolization of cinnamic acids, present in the wine, to their ethyl derivatives. Hence, the participation of two enzymes has been proposed: a p-coumarate decarboxylase (CD) and a vinylphenol reductase (VR). Both enzymes were purified and characterized from B. bruxellensis. In denaturing conditions, the CD enzyme had a molecular mass of 21 kDa, while in native conditions its mass was 41 kDa. The optimal activity was obtained at a temperature of 40 °C and a pH of 6.0. For p-coumaric acid, the Km value and Vmax were 1.22 ± 0.08 mM and 98 ± 0.15 μmol/min mg, respectively. The VR enzyme had a molecular mass of 37 kDa in SDS-PAGE, while in natural conditions its mass was 118 kDa. The Km value was > 3.37 ± 2.05 mM and its Vmax was 107.62 ± 50.38 μmol/min mg for NADPH used as a cofactor. Both enzymatic activities were stable at pH 3.4, but in the presence of ethanol the CD activity decreased drastically while the VR activity was more stable. This is the first report that shows the presence of a CD and a VR enzyme in B. bruxellensis. © 2008 Elsevier B.V. All rights reserved.

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Título según WOS: Purification and characterization of a p-coumarate decarboxylase and a vinylphenol reductase from Brettanomyces bruxellensis
Título según SCOPUS: Purification and characterization of a p-coumarate decarboxylase and a vinylphenol reductase from Brettanomyces bruxellensis
Título de la Revista: INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volumen: 127
Número: 01-feb
Editorial: Elsevier
Fecha de publicación: 2008
Página de inicio: 6
Página final: 11
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0168160508002584
DOI:

10.1016/j.ijfoodmicro.2008.05.011

Notas: ISI, SCOPUS