G alpha q negatively regulates the Wnt-beta-catenin pathway and dorsal embryonic Xenopus laevis development

Soto, X; Mayor, R; Torrejón M.; Montecino, M.; Hinrichs, MV; Olate, J

Abstract

The non-canonical Wnt/Ca2+ signaling pathway has been implicated in the regulation of axis formation and gastrulation movements during early Xenopus laevis embryo development, by antagonizing the canonical Wnt/β-catenin dorsalizing pathway and specifying ventral cell fate. However, the molecular mechanisms involved in this antagonist crosstalk are not known. Since Gαq is the main regulator of Ca2+ signaling in vertebrates and from this perspective probably involved in the events elicited by the non-canonical Wnt/Ca2+ pathway, we decided to study the effect of wild-type Xenopus Gq (xGαq) in dorso-ventral axis embryo patterning. Overexpression of xGαq or its endogenous activation at the dorsal animal region of Xenopus embryo both induced a strong ventralized phenotype and inhibited the expression of dorsal-specific mesoderm markers goosecoid and chordin. Dorsal expression of an xGαq dominant-negative mutant reverted the xGαq-induced ventralized phenotype. Finally, we observed that the Wnt8-induced secondary axis formation is reverted by endogenous xGαq activation, indicating that it is negatively regulating the Wnt/β-catenin pathway. © 2007 Wiley-Liss, Inc.

Más información

Título según WOS: G alpha q negatively regulates the Wnt-beta-catenin pathway and dorsal embryonic Xenopus laevis development
Título según SCOPUS: Gaq negatively regulates the Wnt-ß-catenin pathway and dorsal embryonic Xenopus laevis development
Título de la Revista: JOURNAL OF CELLULAR PHYSIOLOGY
Volumen: 214
Número: 2
Editorial: WILEY-BLACKWELL
Fecha de publicación: 2008
Página de inicio: 483
Página final: 490
Idioma: English
URL: http://doi.wiley.com/10.1002/jcp.21228
DOI:

10.1002/jcp.21228

Notas: ISI, SCOPUS