Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)

Paredes M.; BECERRA V.; Gonzalez Mi

Abstract

Garlic (Allium sativum L.) is a species of vegetative propagation, showing high morphological diversity. Besides, its clones have specific adaptations to different agroclimatic regions. The objective of this study was to determine the genetic diversity of 65 garlic clones collected in Chile and introduced from different countries, by using RAPD (Random Amplified Polymorphic DNA). Fourty random primers of 10 mers generated a total of 398 bands with an 87% of polymorphism. Each primer amplified between two and 20 bands. The size of the fragments obtained fluctuated between 3200 and 369 bp. The results showed that the clones analyzed had a genetic similarity rate of 94%. In addition, 70% of them were clustered in one major group. However, in spite of that situation several clones have different agronomic characteristics

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Título según WOS: Low genetic diversity among garlic (Allium sativum L.) accessions detected using random amplified polymorphic DNA (RAPD)
Título según SCIELO: Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)
Título de la Revista: CHILEAN JOURNAL OF AGRICULTURAL RESEARCH
Volumen: 68
Número: 1
Editorial: INST INVESTIGACIONES AGROPECUARIAS - INIA
Fecha de publicación: 2008
Página de inicio: 3
Página final: 12
Idioma: English
DOI:

10.4067/S0718-58392008000100001

Notas: ISI, SCIELO