Abstract

An alkali and salt-tolerating strain FJAT-44876(T) was isolated from the bauxite residue sample. The 16S rRNA gene sequence and phylogenetic analysis suggest that strain FJAT-44876(T) was a member of the genus Evansella. It grew at 15-45 celcius (optimum 20-25 celcius) and pH 6.5-11.0 (optimum pH 8.0-9.0) with 0-20% (w/v) NaCl (optimum 6-8%). The major fatty acids were anteiso-C-15:0, iso-C-15:0, anteiso-C-17:0, iso-C-17:0, and C-16:0. The cell wall peptidoglycan contained meso-diaminopimelic acid and MK-7 as the menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, and phosphatidylglycerol. The genomic DNA G+C content was 38.2%. The average nucleotide identity values between strain FJAT-44876(T) and closely related members were below the cutoff level for species delineation. Thus, based on the above results, strain FJAT-44876(T) represents a novel species of the genus Evansella, for which the name Evansella halocellulosilytica sp. nov., is proposed. The type strain is FJAT-44876(T) (=CCTCC AB 2016264(T) = DSM 104633(T)).