Abstract

A Gram-stain-negative, rod-shaped, facultatively anaerobic, motile and spore-forming strain designated FJAT-44921(T)was isolated from red mud collected from Chiping County, Shandong Province, China. The 16S rRNA gene sequence result showed that strain FJAT-44921(T)shared a low sequence identity (96.6%) with the members of the genusBacillus. Growth was observed at pH 8.0-10.0 (optimum pH 9.0), 10-40 degrees C (optimum 20-25 degrees C) with 0-8% (v/w %) NaCl (optimum 4-6 v/w %). FJAT-44921(T)consists of MK-7 as the isoprenoid quinone andmeso-2,6-diaminopimelic acid as the cell-wall diamino acid. The predominant fatty acids were anteiso-C-15:0, iso-C-15:0, C-16:0, and anteiso-C-17:0. The polar lipids were diphosphatidylglycerol, phosphatidyl glycerol, phosphatidylmethylethanolamine, unidentified phospholipid, and unidentified aminophospholipid. The genomic DNA G + C content was 37.3 mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between FJAT-44921(T)and other closely relatedBacillusmembers were lower than the recognized threshold values of ANI (95-96%) and dDDH (70%) recommended as the criterion for interspecies identity. The type strain is FJAT-44921(T)(=CCTCC AB 2016196(T) =DSM 104630(T)).