Abstract

A novelBacillusstrain, designated SYSU G01002(T), was isolated from a sediment sample collected from tepid spring in Tengchong, Yunnan province, south-west PR China. The 16S rRNA gene sequence analysis showed that the strain SYSU G01002(T)shared the highest sequence identity with the type strain ofBacillus alkalitolerans(97.7%). Strain SYSU G01002(T)grew at pH 6.0-8.0 (optimum, pH 7.0), at 28-55 degrees C (optimum, 45 degrees C) and in the presence of 0-2.5% (w/v) NaCl (optimum in the absence of NaCl). It containedmeso-2,6-diaminopimelic acid as the cell-wall diamino acid and MK-7 as isoprenoid quinone. The major cellular fatty acids were iso-C(15:0,)iso-C(17:0)and C-16:0. The polar were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and unidentified phospholipid. The genomic DNA G + C content was 38.0 mol %. The digital DNA-DNA hybridization and average nucleotide identity values between SYSU G01002(T)and closely related members of the genusBacilluswere below the cut-off level recommended for interspecies identity. Based on the above results, strain SYSU G01002(T)represents a novel species of the genusBacillus, for which the nameBacillus tepidiphilussp. nov. is proposed. The type strain, SYSU G01002(T)(= KCTC 43131(T) = CGMCC 1.17491(T)).