Lysinibacillus cavernae sp. nov., isolated from cave soil

Kan, Yu; Niu, Xue-Ke; Rao, Manik Prabhu Narsing; Dong, Zhou-Yan; Xie, Yuan-Guo; Kang, Ying-Qian; Li, Wen-Jun

Abstract

A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005(T), was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China. Strain SYSU K30005(T) showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%). In phylogenetic tree, strain SYSU K30005(T) clade with the members of the genus Lysinibacillus. Based on the phylogenetic and 16S gene sequence result, strain SYSU K30005(T) was affiliated to the genus Lysinibacillus. The growth of SYSU K30005(T) was observed at 15-37 degrees C (optimum, 28 degrees C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl). Cell wall peptidoglycan type was A4 alpha (Lys-Asp). The cell-wall sugars of SYSU K30005(T) were ribose, galactose and mannose and MK-7 was the only quinone. The fatty acids (> 5% of total fatty acids) were iso-C-15:0, anteiso-C-15:0, iso-C-16:0 and iso-(C17:0). The polar lipids profile included diphosphatidylglycerol, phosphatideylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The genomic DNA G + C content was 37.2 mol%. The average nucleotide identity values between SYSU K30005(T) and its closest relatives were below the cut-off level (95-96%) for species delineation. The results support the conclusion that strain SYSU K30005(T) represents a novel species of the genus Lysinibacillus, for which we proposed the name Lysinibacillus cavernae sp. nov. The type strain is SYSU K30005(T) (= KCTC 43130(T) = CGMCC 1.17492(T)).

Más información

Título según WOS: ID WOS:000522601100001 Not found in local WOS DB
Título de la Revista: ARCHIVES OF MICROBIOLOGY
Volumen: 202
Número: 6
Editorial: Springer
Fecha de publicación: 2020
Página de inicio: 1529
Página final: 1534
DOI:

10.1007/s00203-020-01852-9

Notas: ISI