Abstract

Two alkaliphilic strains, designated FJAT-45086(T) and FJAT-45122(T), were isolated from alkali soli in Nima County, Tibet, China. Both strains were Gram-positive, rod-shaped and shared low 16S rRNA gene sequence similarity with the members of the genus Bacillus. They contained meso-diaminopimelic acid as the cell-wall diamino acid and MK-7 as the menaquinone. The major fatty acids (>5%) of strain FJAT-45086(T) were anteiso-C-15:0, C-16:0, iso-C-15:0, C-16:1 omega 11c and anteiso-C-17:0,C- whereas strain FJAT-45122(T) consisted of iso-C-15:0, anteiso-C-15:0, iso-C-17:1 omega 10c, iso-C-17:0, anteiso-C-17:0, C-16:0 and C16:1 omega 11c. The genome G+C content of strains FJAT-45086(T) and FJAT-45122(T) was 37.8 and 38.2 mol%, respectively. The polar lipids of strain FJAT-45086(T) were diphosphatidyl glycerol (DPG), phosphatidyl glycerol (PG), phosphatidyl ethanolamine (PE), and phosphatidyl choline (PC), whereas strain FJAT-45122(T) consisted of DPG, PG, phosphatidyl methyl ethanolamine (PME) and an unidentified aminophospholipids (UAPL). The average nucleotide identity values of strains FJAT-45086(T) and FJAT-45122(T) were below the cut-off level (95-96%) for species delineation. Based on the results, strains FJAT-45086(T) and FJAT-45122(T) represent two novel species of the genus Bacillus, for which the names Bacillus alkalisoli sp. nov., and Bacillus solitudinis sp. nov., are proposed. The type strain, FJAT-45122(T) (=DSM 104631(T)=CCTCC AB 2016254(T)), FJAT-45086(T) (=DSM 104056(T)=CCTCC AB 2016232(T)).