Abstract

A Gram-staining-negative, strictly aerobic, non-motile strain, SYSUP0001(T), was isolated from tubers of Gastrodia elata Blume. The 16S rRNA gene sequence result indicated that SYSUP0001(T) represents a member of the genus Sphingomonas, with the highest sequence similarity (97.7%) to the type strain of Sphingomonasginsengisoli. SYSUP0001(T) grew at 14-37 degrees C and pH 6-8, with optimum growth at 28 degrees C and pH 7. Tolerance to NaCl was up to 3% (w/v) with optimum growth in the absence of NaCl. The respiratory quinone was Q-10. The major fatty acids were C-18 (:) (1)omega 7c, Summed feature 3 (C-16 (:) (1)omega 7c/C-16 (:) (1)omega 6c), and C-16 (: 0). The polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), sphingoglycolipid (SGL), phosphatidylcholine (PC) and four unidentified polar lipids (L). The DNA G+C content was 67.5 %. The average nucleotide identity (ANI) values between SYSUP0001 T and closely related members of the genus Sphingomonas were below the cut-off level (95-96 %) for species delineation. On the basis of the phenotypic, phylogenetic and chemotaxonomic characterizations, SYSUP0001 T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas mesophila sp. nov. is proposed. The type strain is SYSUP0001(T) (=KCTC 62179(T) =CGMCC 1.16462(T)).