Abstract

A Gram-stain-positive, rod-shaped, motile bacterium designated as SYP-B691(T) was isolated from rhizospheric soil of Panax notoginseng. Phylogenetic analysis indicated that SYP-B691(T) clearly represented a member of the genus Bacillus and showed 16S rRNA gene similarity lower than 97.0% with the type strains of species of the genus Bacillus, which indicates that it should be considered as a candidate novel species within this genus. The optimum growth of the strain was found to occur at 37 degrees C and pH 7.0-9.0. The genomic DNA G+C content was determined to be 45.2 mol%. It contained meso-2,6-diaminopimelic acid in the cell-wall peptidoglycan. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. MK-7 was the only menaquinone identified. The major cellular fatty acids of SYP-B691(T) were identified as iso-C15:0 and anteiso-C15:0. On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, SYP-B691(T) merits recognition as a representative of a novel species of the genus Bacillus, for which the name Bacillus notoginsengisoli sp. nov. is proposed, with SYP-B691(T) (= DSM 29196(T) = JCM 30743(T)) as the type strain.