Abstract

A Gram-stain-positive actinobacterium, designated strain YIM DR4008(T), was isolated from the root sample of Psammosilene tunicoides collected from Lijiang, Yunnan, China. Strain YIM DR4008T could grow at temperatures ranging from 10 to 50 degrees C (optimum 28-30 degrees C), at pH 5.0-11.0 (optimum pH 7.0) and in the presence of up to 4% (w/v) NaCl. Sequence analysis of the 16S ribosomal RNA gene revealed that strain YIM DR4008(T) shared highest similarity (95.0 %) with Streptomyces griseoplanus NBRC 12779(T) and <95% similarity with other known members of the genera Streptomyces, Kitasatospora and Streptacidiphilus. The diagnostic cell-wall diamino acid of strain YIM DR4008(T) was found to be LL-diaminopimelic acid. The whole-cell hydrolysates contained a major amount of galactose and mannose along with a small proportion of fucose, glucose, rhamnose and ribose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylinositol mannosides and three unidentified phospholipids. The respiratory menaquinones were MK-9(H-6) and MK-9(H-8), while the major cellular fatty acids (> 10 %) were anteiso-C-15:0, C-16: 0, iso-C-16: 0, iso-C-15: 0 and anteiso-C-17: 0. The genomic DNA G+C content was determined to be 75.3 mol%. Based on the phenotypic, chemotaxonomic and molecular characteristics, strain YIM DR4008(T) is proposed to be recognized as a novel species of a new genus in the family Streptomycetaceae, with the name Allostreptomyces psammosilenae gen. nov., sp. nov. The type strain of the type species is YIM DR4008(T) (=DSM 42178(T) = CGMCC 4.7247(T)). An emended description of the family Streptomycetaceae is also provided.