Abstract

A novel endophytic actinobacterial strain, designated EGI 6500195(T), was isolated from fruits of Capparis spinosa. Growth occurred at 10-45 degrees C (optimum 30 degrees C), at pH 6-8 (optimum pH 7) and in the presence of 0-1% (w/v) NaCl. Strain EGI 6500195(T) shared highest 16S rRNA gene sequence similarity (97.74 %) with Streptomyces vitaminophilus DSM 41686(T) and less than 97% sequence similarity with other members of the genus Streptomyces. The diagnostic amino acid in the peptidoglycan was LL-diaminopimelic acid. Whole-cell hydrolysates contained glucose, ribose, fructose and mannose. The predominant menaquinones were MK-9(H-6/) and MK-9(H-8). The polar lipid profile of strain EGI 6500195(T) included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylcholine, three unknown phospholipids, an unknown aminophospholipid and an unknown aminolipid. The cellular fatty acids were anteiso-C-15:0, anteiso-C-17:0, iso-C-15:0, iso-C-16:0, anteiso-C-17:1 omega 9c, summed feature 4 (iso-C-17:1 I and/or anteiso-C-17:1 B) and iso-C-17:1 omega 9c. The DNA G+C content of strain EGI 6500195(T) was 74.1 mol%. The level of DNA-DNA relatedness between strain EGI 6500195T and Streptomyces. vitaminophilus DSM 41686(T) was 14.1 +/- 3.5 %. On the basis of the phenotypic, phylogenetic, chemotaxonomic and DNA-DNA hybridization data, strain EGI 6500195T represents a novel species of the genus Streptomyces, for which the name Streptomyces capparidis sp. nov. is proposed. The type strain is EGI 6500195(T) (= DSM 42145(T)= JCM 30089(T)).