Review ISI SCOPUS
Journal of Chromatography A  (2009)

Methods of calculating protein hydrophobicity and their application in developing correlations to predict hydrophobic interaction chromatography retention

Mahn A.; Salgado JC

Abstract

Hydrophobic interaction chromatography (HIC) is a key technique for protein separation and purification. Different methodologies to estimate the hydrophobicity of a protein are reviewed, which have been related to the chromatographic behavior of proteins in HIC. These methodologies consider either knowledge of the three-dimensional structure or the amino acid composition of proteins. Despite some restrictions; they have proven to be useful in predicting protein retention time in HIC. © 2008 Elsevier B.V. All rights reserved.

Más información

Título según WOS: Methods of calculating protein hydrophobicity and their application in developing correlations to predict hydrophobic interaction chromatography retention
Título según SCOPUS: Methods of calculating protein hydrophobicity and their application in developing correlations to predict hydrophobic interaction chromatography retention
Título de la Revista: Journal of Chromatography A
Volumen: 1216
Número: 10
Editorial: Elsevier B.V.
Fecha de publicación: 2009
Página de inicio: 1838
Página final: 1844
Idioma: eng
URL: http://linkinghub.elsevier.com/retrieve/pii/S0021967308021250
DOI:

10.1016/j.chroma.2008.11.089
Notas: ISI, SCOPUS