Gamma-Linolenic and Pinolenic Acids Exert Anti-Inflammatory Effects in Cultured Human Endothelial Cells Through Their Elongation Products

Baker, Ella J.; Valenzuela, Carina A.; van Dooremalen, Wies T. M.; Martinez-Fernandez, Leyre; Yaqoob, Parveen; Miles, Elizabeth A.; Calder, Philip C.

Abstract

Scope Omega-3 fatty acids (FAs) from oily fish reduce cardiovascular disease. This may be partly due to modulation of endothelial cell (EC) inflammation. Fish stocks are declining and there is a need for sustainable alternative FAs. Gamma-linolenic acid (GLA) and pinolenic acid (PLA) are plant-derived FAs, which can fulfil this role. Methods and results EA.hy926 cells are exposed GLA and PLA prior to stimulation with tumor necrosis factor (TNF)-alpha. GLA and PLA are incorporated into ECs, resulting in increases in long-chain derivatives produced by elongase 5, dihomo-gamma-linolenic acid (DGLA), and eicosatrienoic acid (ETA). Both GLA and PLA (50 mu m) decrease production of soluble intercellular adhesion molecule-1 (sICAM-1), monocyte chemoattractant protein 1 (MCP-1), and regulated on activation, normal T cell expressed and secreted (RANTES). However, decreases in these mediators are not seen after pre-treatment with GLA or PLA in elongase 5 silenced EA.hy926 cells. DGLA and ETA (10 mu m) decrease EC production of sICAM-1, MCP-1, RANTES, and IL-6. All FAs reduce adhesion of THP-1 monocytes to EA.hy926 cells. Both PLA (50 mu m) and ETA (10 mu m) decrease NF kappa Bp65 phosphorylation. Conclusion These effects suggest potential for GLA, PLA and their long-chain derivatives, DGLA and ETA, as sustainable anti-inflammatory alternatives to fish-derived FAs.

Más información

Título según WOS: Gamma-Linolenic and Pinolenic Acids Exert Anti-Inflammatory Effects in Cultured Human Endothelial Cells Through Their Elongation Products
Título de la Revista: MOLECULAR NUTRITION & FOOD RESEARCH
Volumen: 64
Número: 20
Editorial: Wiley
Fecha de publicación: 2020
DOI:

10.1002/mnfr.202000382

Notas: ISI