Abstract

Objective: Obesity is associated with liver depletion of cd-3 polyunsaturated fatty acids (cd-3 PUFAS) promoting steatosis and inflammation, whose levels are maintained by diet or biosynthesis involving 0-5D, 0-6D desaturases and elongases. Method: We aimed to assess 0-5D and 0-6D activities in liver and brain from mice fed a control diet (CD) or high-fat diet (HFD) for four to sixteen weeks. Results: HFD led to (1) an early (4 weeks) enhancement in liver 0-5D, 0-6D, and PPAR-a activities, without changes in oxidative stress, liver damage or fat accumulation; (2) a latter progressive loss in hepatic desaturation with insufficient compensatory increases in mRNA and protein expression, leading to cd-3 PUFA depletion, PPAR-a down-regulation reducing FA oxidation, and liver steatosis with enhancement in lipogenesis; and (3) brain cd-3 PUFA depletion after 12 to 16 weeks of HFD feeding. Conclusion: In conclusion, the brain-liver axis is drastically affected by obesity in a time dependent fashion. (c) 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.