Double immunostaining for p53 and molecular chaperone hsp72/73 in gastric carcinoma

Villaseca M.A.; Roa, I; Araya J.C.; Roa, J.C; Flores P.

Keywords: proteins, localization, expression, protein, cell, heat, nucleus, carcinoma, immunohistochemistry, tumor, humans, human, male, aged, peroxidase, p53, tissue, neoplasms, adult, female, shock, stomach, article, heat-shock, cytoplasm, suppressor, neoplasm, techniques, controlled, clinical, study, streptavidin, priority, middle, journal, and, Aged,, 80, over, Immunoenzyme, biotin, HSP72

Abstract

Aims - To examine the relation between the expression of p53 protein and the chaperone heat shock protein (hsp) 72/73 in a population at high risk for gastric carcinoma, suing single and double immunohistochemistry, and to compare the expression of these two proteins with clinicopathological features. Methods - Monoclonal antibodies were used to investigate the expression of p53 protein and hsp72/73 in 46 human gastric carcinomas. A double immunohistochemistry technique was used in cases that showed p53/hsp72/73 coexpression. Results - p53 immunoreactvity was present in 11 tumours (24%), and hsp72/73 immunostaining was observed in 22 cases (48%). p53 expression was observed as nuclear staining in tumoral cells. hsp72/73 expression was demonstrated mainly as cytoplasmic staining, but six tumours also showed focal weak nuclear staining. Seven cases showed p53 and hsp72/73 coexpression with immunoreactivity for both proteins in the same neoplastic cells, three of them with focal areas of nuclear co-expression. p53 expression was seen more frequently in cases that showed a high intensity (+++) of hsp72/73 staining. No significant association was observed between the expression of the two proteins and clinicopathological features. Conclusions - More than half of our cases may have some impairment in p53 protein growth suppressive function, as a result of p53 gene alterations or complex formation. The positive correlation between p53 expression and intensity of hsp72/73 supports the postulate of a p53 regulating function for the chaperone hsp72/73. A high intensity of hsp72/73 immunohistochemical staining could be used as an indirect marker of p53 gene abnormalities.

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Título de la Revista: Journal of Clinical Pathology - Molecular Pathology
Volumen: 50
Número: 6
Editorial: Society of Laparoendoscopic Surgeons
Fecha de publicación: 1997
Página de inicio: 317
Página final: 321
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-0031455949&partnerID=q2rCbXpz