Molecular identification of four species of mussels from southern Chile by PCR-based nuclear markers: The potential use in studies involving planktonic surveys

Toro J.E.

Keywords: chile, structure, chain, mytilus, polymerase, molecular, edulis, cellular, purpuratus, choromytilus, Reaction, Perumytilus, and, Aulacomya, ater, chorus

Abstract

PCR amplification of two nuclear DNA fragments (ITS and GLU-5) and subsequent restriction fragment polymorphism (RFLP) were applied to mantle tissue samples from four species of Chilean mussels, Mytilus edulis chilensis, Choromytilus chorus, Aulacomya ater, and Perumytilus purpuratus, and to individual D-shape larvae of Mytilus edulis and M. trossulus from Newfoundland, in order to look for specific banding patterns for each of the Chilean species. Both nuclear DNA-based markers produced species-specific banding patterns and may therefore be able to differentiate larvae from the four Chilean mussels during studies that rely on plankton surveys.

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Título de la Revista: JOURNAL OF SHELLFISH RESEARCH
Volumen: 17
Número: 4
Editorial: NATL SHELLFISHERIES ASSOC
Fecha de publicación: 1998
Página de inicio: 1203
Página final: 1205
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-0032421110&partnerID=q2rCbXpz